Trempealeau, Wisconsin is located on the eastern bank of the Mississippi River, just 18 miles upriver of La Crosse, WI and down river of Winona, MN. The Village of Trempealeau rests on high rolling banks at the edge of the river, smack in the center of a stretch of Wisconsin State Highway 35, known as the Great River Road, voted one of the ten most scenic drives in America.
